Office of Environmental Health Hazard Assessment

Water

Update of the Public Health Goal for 1,3-Dichloropropene
[12/22/06]

download the memo for Telone here

Under the Calderon-Sher California Safe Drinking Water Act of 1996, the Office of Environmental Health Hazard Assessment (OEHHA) develops public health goals (PHGs) for regulated chemicals in drinking water and reviews and updates the risk assessments every five years (Health and Safety Code Section 116365(e)(1). This memorandum represents an update of the literature review and reevaluation of the existing PHG for 1,3-dichloropropene, also known as Telone II® (OEHHA, 1999). Our re-evaluation supports the previous PHG derivation in 1999. We conclude that the PHG for 1,3-dichloropropene should remain at 0.2 parts per billion (ppb).

Summary of Review
We have surveyed the scientific literature for recently published research studies to determine if there are new toxicity studies that would warrant revising the PHG of 0.2 ppb or making substantive changes to the PHG support document. We also searched for new risk assessments of 1,3-dichloropropene since the publication of the PHG document in 1999, including U.S. EPA reviews, and new risk assessment methods that might be applied to evaluation of 1,3-dichloropropene.

No new studies were found that affect the choice of the critical studies used as the basis for the existing PHG value. Risk assessment methods for consideration of the effect of early-life exposures on carcinogenic potency are under consideration, but OEHHA guidelines have not yet been developed. No new data were found that could provide any further important insight on the toxic effects of 1,3-dichloropropene, data that could change the existing approach to the PHG establishment and/or shed more light on carcinogenicity of this compound. The following sections provide brief summaries of the current status of health risk evaluations of 1,3-dichloropropene that are pertinent to the establishment of our PHG.

Chronic Toxicity
The lowest No-Observed-Adverse-Effect Level (NOAEL) of 2.5 mg/kg-day identified by OEHHA in 1999 still remains the most appropriate value for quantitative risk assessment of noncarcinogenic effects caused by 1,3-dichloropropene (OEHHA, 1999). This NOAEL was established in rats (Stott et al., 1995), with support from studies in mice (Redmond et al., 1995) and dogs (Stott et al., 1992). The NOAEL in rats was based on a significant decrease in body weights and a dose-related increase in basal cell hyperplasia of the non-glandular stomach mucosa of both males and females at 12.5 and 25 mg/kg-day. The LOAEL identified in this study was 12.5 mg/kg-day. In mice, the LOAEL was 25 mg/kg-day based on decreased body weights and body weight gains in males and females. The LOAEL in the dog study was 15 mg/kg-day based on an increase in hematopoiesis in bone marrow and extramedullary hematopoiesis in the spleen, consistent with a regenerative response to hypochromic, microcytic anemia. The health-protective level based on noncarcinogenic effects resulting from chronic exposures to 1,3- dichloropropene is 90 ppb.

Carcinogenicity
Two-year animal bioassays demonstrate carcinogenicity of 1,3-dichloropropene. Feeding studies in rodents by Stott et al. (1995) showed an increase in the incidence of benign hepatocellular adenomas (with one hepatocarcinoma) in male rats at 25 mg/kg-day, the highest dose tested. No treatment-related tumors were observed in female rats or male or female mice fed up to 50 mg/kg-day (Stott et al., 1995; Redmont et al., 1995). However, the thrice weekly gavage study by NTP (1985) found significant incidences of bronchioalveolar, forestomach, and urinary bladder tumors in mice at 50 mg/kg and forestomach and liver tumors in rats at 25 mg/kg. With the exception of urinary bladder tumors in mice, most tumors were benign. In rats at 50 mg/kg, four carcinomas were observed in the forestomach and one was observed in the liver. In mice, eight carcinomas in the urinary bladder and three in bronchioalveolar areas were observed at 50 mg/kg, while two were found in the forestomach at 100 mg/kg. The microencapsulated 1,3-dichloropropene used in the study of Stott et al. (1995) did not contain
the 1% epichlorohydrin which was used as a stabilizer in the earlier NTP gavage study (U.S. EPA, 1998). Because epichlorohydrin caused hyperplasia, papillomas and carcinomas in the forestomach of rats in a drinking water study (Konishi et al., 1980), it has been hypothesized that epichlorohydrin may be partially responsible for the squamous cell papillomas and carcinomas, at least in the rat forestomach. The chronic feeding study by Stott et al. (1995), which did not include epichlorohydrin, found forestomach hyperplasia in rats but no carcinomas or papillomas.

Mutagenic and Genetic Toxicology
The mutagenicity and genotoxicity of 1,3-dichloropropene have been studied in both in vitro and in vivo assays (U.S. EPA, 2000). Early bacterial studies found mutagenic activity for 1,3-dichloropropene in a variety of test systems in the absence of metabolic activation. Even though later studies showed that these findings were due to mutagenic impurities in the 1,3- dichloropropene formulations, purified 1,3-dichloropropene produced mutations in the presence of S9.

1,3-dichloropropene was found to be a relatively potent inducer of unscheduled DNA synthesis (UDS) in comparison to other tested allylic compounds (cis- >trans-1,3-dichloropropene) (Schiffmann et al., 1983). 1,3-DCP exposure resulted in the induction of sister chromatid exchange in vitro in human lymphocytes with and without addition of a metabolic activation system (Kevekordes et al., 1996).

Usage of Telone in California
The trend of increasing use (see figure 1) for 1,3-dichloropropene in California (Fan and Walters, 2005) as methyl bromide is phased out is likely to result in increasing exposures to this chemical in air. The possibility for groundwater contamination as use increases also should be considered, although in the most recent agricultural well sampling results, no 1,3-dichloropropene was detected (DPR, 2005a). The statewide total amount of 1,3- dichloropropene use doubled between the years 2000 and 2004, from 4.4 million lbs/year to over 8.9 million lbs/year. The use of 1,3-dichloropropene in California is controlled by a management plan (DPR, 2002) to limit the health risk from exposure, especially by the inhalation route. Air monitoring for this chemical was conducted in Ventura County in 2005, and may be continued in 2006 (DPR, 2005b).

Review of the Existing PHG Value
As stated in the 1999 PHG document for 1,3-dichloropropene, the critical concern is carcinogenicity, and the most important issue for the risk assessment is deriving the carcinogenic potency factor. At this time, no studies exist which would either change the carcinogenic determination or provide a basis for a revised potency factor. U.S. EPA does not currently regulate 1,3-dichloropropene in drinking water, but has established a Health Advisory level of 0.2 ppb, based on a 10-6 cancer risk (U.S. EPA, 1998). Establishment of the OEHHA PHG was also based on carcinogenicity, in the two oral studies (NTP 1985, Stott et al., 1995). Our present review confirms this earlier determination.

After finalization of the new cancer guidelines (U.S. EPA, 2005a) and the supplemental guidance for early-life exposures (U.S. EPA, 2005b) by U.S. EPA, OEHHA is currently reviewing its procedures for assessing cancer potency, especially to ensure adequate protection of sensitive subpopulations. The U.S. EPA has proposed increasing cancer potency values that are based on animal studies which did not incorporate early-life exposures by specific amounts to allow for infant and child exposures to the chemicals (U.S. EPA, 2005b). This requires some judgment concerning how to apply the principles to specific types of chemicals in drinking water. Discussion of these factors is presently underway at OEHHA, and in the meantime, the traditional approach is being used. From the perspective of noncancer risk determinations, no new studies exist which would be more appropriate to replace the one selected for the noncancer assessment in the existing PHG document. For all these reasons, OEHHA concludes that the PHG of 0.2 ppb is adequate to protect sensitive subpopulations, including pregnant women and their fetuses, infants, and the elderly.

References
Bartels MJ, Brzak KA, Mendrala AL, Stott WT (2000). Mechanistic aspects of the metabolism of 1,3-dichloropropene in rats and mice. Chem Res Toxicol 13(11):1096-102.

DPR (2002). California Management Plan: 1,3-Dichloropropene. California Department of Pesticide Regulation, Sacramento, CA (January). Accessed at: http://www.cdpr.ca.gov/docs/empm/pubs/tac/tac_regs.htm

DPR (2005a) Sampling for Pesticide Residues in California Well Water. California Department of Pesticide Regulation, Sacramento, CA (December). Accessed at: http://www.cdpr.ca.gov/docs/wellinv/wirmain.htm

DPR (2005b). Status report for fumigant pesticides. California Department of Pesticide Regulation, Sacramento, CA (October). Accessed at: http://www.cdpr.ca.gov/docs/dprdocs/methbrom/stat1005.pdf

DPR (2006). Summary of Pesticide Use Report Data 2004, Indexed by Chemical. California Department of Pesticide Regulation, Sacramento, CA. Accessed at: http://www.cdpr.ca.gov/docs/pur/pur04rep/04_pur.htm.

Fan S, Walters J (2005). Use Information and Ambient Air Monitoring Recommendations for Fumigations with the Pesticide Active Ingredients 1,3-Dichloropropene and Methyl Bromide. California Department of Pesticide Regulation, Sacramento, CA. Accessed at: http://www.cdpr.ca.gov/docs/empm/pubs/tac/
recomm/telonemebr_rec05.pdf

Gollapudi BB, Ciszlak FS, Day SJ, Carney EW (1998). Dominant lethal test with rats exposed to 1,3-dichloropropene by inhalation. Environ Mol Mutagen 32(4):351-9.

Haber LT, Maier A, Zhao Q, Dollarhide JS, Savage RE, Dourson ML (2001). Applications of mechanistic data in risk assessment: the past, present, and future. Toxicol Sci 61(1):32-9.

IARC (1999). 1,3-Dichloropropene. International Agency for Research on Cancer (IARC) Monogr Eval Carcinog Risk Hum 71 Pt 3:933-45.

Kevekordes S, Gebel T, Pav K, Edenharder R, Dunkelberg H (1996). Genotoxicity of selected pesticides in the mouse bone-marrow micronucleus test and in the sister-chromatid exchange test with human lymphocytes in vitro. Toxicol Lett 89(1):35-42.

Konishi Y, Kawabata A, Denda A, Ikeda T, Katada H, Maruyama H, et al. (1980). Forestomach tumors induced by orally administered epichlorohydrin in male Wistar rats. Gann 71:922-3.

Lee S, McLaughlin R, Harnly M, Gunnier R, Kreutzer R (2002). Community exposures to airborne agricultural pesticides in California: ranking of inhalation risks. Environ Health Perspect 110(12):1175-84.

NTP (1985). Toxicology and carcinogenesis studies of Telone II (technical grade 1,3-dichloropropene [CAS No. 542-75-6] containing 1% epichlorohydrin as a stabilizer) in F344/N rats and B6C3F1 mice (gavage studies). NTP TR 269. National Toxicology Program, Research Triangle Park, NC.

Office of Environmental Health Hazard Assessment (1999). Public Health Goal for 1,3-Dichloropropene in Drinking Water. California Environmental Protection Agency, Sacramento, CA.

Poelarends GJ, Kulakov LA, Larkin MJ, van Hylckama Vlieg JE, Janssen DB (2000). Roles of horizontal gene transfer and gene integration in evolution of 1,3-dichloropropene- and 1,2-dibromoethane-degradative pathways. J Bacteriol 182(8):2191-9.

Redmond JM, Stebbins KE, Stott WT (1995). Telone II soil fumigant: Two-year dietary chronic toxicity/oncogenicity study in B6C3F1 mice – final report. Study ID: M-003993032. The Dow Chemical Co., Midland, MI. August 9, 1995.

Schneider M, Quistad GB, Casida JE (1998). 1,3-Dichloropropene epoxides: intermediates in bioactivation of the promutagen 1,3-dichloropropene. Chem Res Toxicol 11(10):1137-44.

Secondin L, Maso S, Trevisan A (1999). Different effects of (cis+trans) 1,3-dichloropropene in renal cortical slices derived from male and female rats. Hum Exp Toxicol 18(2):106-10.

Stebbins KE, Johnson KA, Jeffries TK, Redmond JM, Haut KT, Shabrang SN, Stott WT (2000). Chronic toxicity and oncogenicity studies of ingested 1,3-dichloropropene in rats and mice. Reg Toxicol Pharmacol 32(1):1-13.

Stebbins KE, Quast JF, Haut KT, Stott WT (1999). Subchronic and chronic toxicity of ingested 1,3-dichloropropene in dogs. Regul Toxicol Pharmacol 30 (3):233-43.

Stott WT, Johnson KA, Jeffries TK, Haut KT, Shabrang SN (1995). Telone II soil fumigant: Two-year chronic toxicity/oncogenicity study in Fisher 344 rats. Study ID: M-003993-031. Toxicology Research Laboratory, Dow Chemical Co., Midland MI. August 15, 1995.

Stott WT, Stebbins KE, Haut KT, Quast JF, Shabrang SN (1992). Telone II soil fumigant: One year dietary toxicity study in beagle dogs. Dow Chemical Co., Midland MI. July 22, 1992. Study ID: M-003993-024.

U.S. EPA (1998). Reregistration Eligibility Decision (RED): 1,3-Dichloropropene. U.S. Environmental Protection Agency, Washington, DC. Accessed at: http://www.epa.gov/REDs/0328red.pdf.

U.S. EPA (2000). Toxicological Review of 1,3-Dichloropropene. U.S. Environmental Protection Agency, Washington, DC. Accessed at: http://www.epa.gov/iris/toxreviews/0224-tr.pdf.

U.S. EPA (2005a). Guidelines for Carcinogen Risk Assessment. Risk Assessment Forum, U.S. Environmental Protection Agency, Washington, DC. EPA/630/P-03/001B. Accessed at: http://cfpub.epa.gov/ncea/cfm/recordisplay.cfm?deid=116283.
U.S. EPA (2005b). Supplemental Guidance for Assessing Susceptibility from Early-life Exposure to Carcinogens. Risk Assessment Forum, U.S. Environmental Protection Agency, Washington, DC. EPA/630/R-03/003F. Accessed at: http://cfpub.epa.gov/ncea/cfm/recordisplay.cfm?deid=116283.

Verplanke AJ, Bloemen LJ, Brouwer EJ, Van Sittert NJ, Boogaard PJ, Herber RF, De Wolf FA (2000). Occupational exposure to cis-1,3-dichloropropene: biological effect monitoring of kidney and liver function. Occup Environ Med 57(11):745-51.

Wang Q, Gan J, Papiernik SK, Yates SR (2001). Isomeric effects on thiosulfate transformation and detoxification of 1,3-dichloropropene. Environ Toxicol Chem 20(5):960-4

 

 
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